PESQUISA VETERINÁRIA BRASILEIRA

Abstract in English:

ABSTRACT.- Zuccari D.A.P.C, Castro R., Gavioli A.F., Mancini U.M., Frade C.S., Pivaro L.R., Carmona-Raphe J., Terzian A.C.B., Ruiz C.M., Goloni-Bertollo E.M., Pavarino-Bertelli E.C. & Tajara E.H. 2009. The maspin expression in canine mammary tumors: An immunohistochemical and molecular study. Pesquisa Veterinária Brasileira 29(2):167-173. Centro Regional de Bioterismo, Faculdade de Medicina de São José do Rio Preto, Av. Brigadeiro Faria Lima 5416, São José do Rio Preto, SP 15090-000, Brazil. E-mail: debora.zuccari@famerp.br The serpin maspin, a tumor suppressor in breast cancer was described as an inhibitor of cell migration and inducer of cell adhesion between the basement membrane and extracellular matrix resulting in inhibition of tumor metastasis. In contrast, overexpression of maspin is correlated with poor prognosis in other types of cancer. Little is known about expression, regulation and function of maspin in canine mammary tumors. It was demonstrated in this study, a loss of maspin expression in malignant canine mammary cells compared with a pool of normal canine mammary tissue, analyzed by quantitative real-time PCR; weak maspin expression in malignant canine mammary tumors were observed by immunohistochemistry. It was also demonstrated that a correlation with nuclear maspin expression and a good prognosis. It is suggested that maspin could be used as a prognostic marker in canine mammary neoplasia.

• Maspin canine mammary tumor immunohistochemistry molecular biology prognosis

Abstract in Portuguese:

ABSTRACT.- Zuccari D.A.P.C, Castro R., Gavioli A.F., Mancini U.M., Frade C.S., Pivaro L.R., Carmona-Raphe J., Terzian A.C.B., Ruiz C.M., Goloni-Bertollo E.M., Pavarino-Bertelli E.C. & Tajara E.H. 2009. The maspin expression in canine mammary tumors: An immunohistochemical and molecular study. Pesquisa Veterinária Brasileira 29(2):167-173. Centro Regional de Bioterismo, Faculdade de Medicina de São José do Rio Preto, Av. Brigadeiro Faria Lima 5416, São José do Rio Preto, SP 15090-000, Brazil. E-mail: debora.zuccari@famerp.br The serpin maspin, a tumor suppressor in breast cancer was described as an inhibitor of cell migration and inducer of cell adhesion between the basement membrane and extracellular matrix resulting in inhibition of tumor metastasis. In contrast, overexpression of maspin is correlated with poor prognosis in other types of cancer. Little is known about expression, regulation and function of maspin in canine mammary tumors. It was demonstrated in this study, a loss of maspin expression in malignant canine mammary cells compared with a pool of normal canine mammary tissue, analyzed by quantitative real-time PCR; weak maspin expression in malignant canine mammary tumors were observed by immunohistochemistry. It was also demonstrated that a correlation with nuclear maspin expression and a good prognosis. It is suggested that maspin could be used as a prognostic marker in canine mammary neoplasia.

Abstract in English:

ABSTRACT.- Zuccari D.A.P.C., Pavam M.V., Terzian A.C.B., Pereira R.S., Ruiz C.M. & Andrade J.C.† 2008. Immunohistochemical evaluation of Ki-67 and PCNA in canine mammary neoplasias: Correlation with prognostic factors and clinical outcome. Pesquisa Veterinária Brasileira 28(4):207-215. Centro Regional de Bioterismo, FAMERP, Av. Brigadeiro Faria Lima 5416, São José do Rio Preto, SP 15090-000, Brazil. E-mail: debora.zuccari@famerp.br E-cadherin is a cell-cell adhesion molecule and low e-cadherin expression is related to invasiveness and may indicate a bad prognosis in mammary neoplasms. The expression of cell proliferation markers PCNA and especially Ki-67, has also proved to have a strong prognostic value in this tumor class. The expression of these markers was related to the clinical-pathological characteristics of 73 surgically removed mammary tumors in female dogs by immunohistochemistry. There was no statistical correlation between these markers and death by neoplasm, survival time and disease-free interval. However, the loss of e-cadherin expression and marked Ki-67 expression (p=0.016) were considered statistically significant for the diagnosis (p=0.032). When evaluated as independent factors, there was evidence of the relationship between the loss of e-cadherin expression and high PCNA expression with changes in the body status (divided into obese, normal and cachectic) of female dogs (p=0.030); there was also evidence of the relationship between pseudopregnancy and e-cadherin alone (p=0.021) and for ulceration and PCNA alone (p=0.035). The significant correlation between the markers expression and these well known prognostic factors used individually or in combination suggests their prognostic value in canine mammary tumors.

• Canine cell proliferation marker e-cadherin immunohistochemistry mammary neoplasm

Abstract in Portuguese:

ABSTRACT.- Zuccari D.A.P.C., Pavam M.V., Terzian A.C.B., Pereira R.S., Ruiz C.M. & Andrade J.C.† 2008. Immunohistochemical evaluation of Ki-67 and PCNA in canine mammary neoplasias: Correlation with prognostic factors and clinical outcome. Pesquisa Veterinária Brasileira 28(4):207-215. Centro Regional de Bioterismo, FAMERP, Av. Brigadeiro Faria Lima 5416, São José do Rio Preto, SP 15090-000, Brazil. E-mail: debora.zuccari@famerp.br E-cadherin is a cell-cell adhesion molecule and low e-cadherin expression is related to invasiveness and may indicate a bad prognosis in mammary neoplasms. The expression of cell proliferation markers PCNA and especially Ki-67, has also proved to have a strong prognostic value in this tumor class. The expression of these markers was related to the clinical-pathological characteristics of 73 surgically removed mammary tumors in female dogs by immunohistochemistry. There was no statistical correlation between these markers and death by neoplasm, survival time and disease-free interval. However, the loss of e-cadherin expression and marked Ki-67 expression (p=0.016) were considered statistically significant for the diagnosis (p=0.032). When evaluated as independent factors, there was evidence of the relationship between the loss of e-cadherin expression and high PCNA expression with changes in the body status (divided into obese, normal and cachectic) of female dogs (p=0.030); there was also evidence of the relationship between pseudopregnancy and e-cadherin alone (p=0.021) and for ulceration and PCNA alone (p=0.035). The significant correlation between the markers expression and these well known prognostic factors used individually or in combination suggests their prognostic value in canine mammary tumors.